FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154512
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32400.046.836821
TATCAAC37450.040.3987852
GGTATCA15800.038.7408871
GTGGTAT7300.038.4313161
ATCAACG39200.038.268373
TCAACGC39350.038.1224944
CAACGCA40250.037.3756455
AACGCAG41400.036.2347876
ACGCAGA41900.035.7009667
CGCAGAG43700.034.327698
TGGTATC8200.032.1327552
GCAGAGT48950.030.81969
CGGTTAA755.8996835E-428.3382431
GTACGGA1251.7484146E-523.8041231
CAGAGTA43100.023.56626510-11
AGAGTAC40850.022.10736710-11
GAGTACG49700.020.26659812-13
GTACGGG50600.019.69614614-15
AGTACGG46250.019.34324312-13
ATATATA1559.19615E-519.1927192
Produced by FastQC (version 0.11.5)