FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154512
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25000.21654170766522998No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29450.029.2399221
GGTATCA14300.028.6660211
GTGGTAT6000.027.5116831
TGGTATC6350.026.4113732
TATCAAC33950.025.5097142
TCAACGC34450.025.131854
ATCAACG35050.024.8606223
CAACGCA35200.024.5974355
TATTAGA2400.024.0570772
AACGCAG35900.023.9657296
GTATTAG3500.023.5814441
ACGCAGA36900.023.3162547
AGAGTAC36600.023.21500211
CGCAGAG37150.023.1603498
CAGAGTA38700.022.2385210
GCAGAGT41950.020.6440359
AGTACGG41400.020.45876113
GTCGGAC700.004975049319.64353815
GAGTACG44100.019.2053512
TACGGGC5700.018.81644215
Produced by FastQC (version 0.11.5)