FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746460
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31180.1785325744649176No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT30170.17274944745370635No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.13598937278838336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13350.042.3406372
GTATCAA58400.041.6348721
GTGGTAT14100.041.302421
GGTATCA30200.038.5671651
TATCAAC63550.038.3200532
TCAACGC67350.036.09284
ATCAACG67450.035.9762843
CAACGCA68300.035.6535
AACGCAG71250.034.296136
CGCAGAG76500.031.830468
ACGCAGA77800.031.5170767
GCAGAGT83500.029.1620399
CAGAGTA61200.024.92830510-11
GAGTACG68900.021.86486212-13
AGAGTAC57850.021.26644510-11
GTCCTAA3400.020.0039221
GTACGGG78050.019.11047614-15
AGTACGG66500.018.37240212-13
GTGTAAG6400.016.6048181
ACGGGAA8400.016.1885516-17
Produced by FastQC (version 0.11.5)