FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746460
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74270.4252602407155045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10800.032.334972
GTATCAA53400.030.2482551
GTGGTAT12100.029.7912981
GGTATCA24850.027.1295851
TATCAAC60150.026.6517262
TCAACGC63400.025.2363574
ATCAACG63800.025.0350483
CAACGCA64950.024.80275
AACGCAG66450.024.4917436
AGAGTAC65050.024.22073611
CGCAGAG68150.023.719448
CAGAGTA67150.023.58476810
TACGCGA1052.1016967E-723.56924429
ACGCAGA69600.023.2647847
GCAGAGT72150.022.5594259
AGTACGG74000.020.99768313
GAGTACG74850.020.90440412
TACGGGG29700.019.72378215
TGCCGAC6750.019.55934355
TAGGTCG856.7015976E-419.4049635
Produced by FastQC (version 0.11.5)