FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289317
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17480.13557565749928063No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT16560.12844009657826586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38900.043.1641961
GTGGTAT9950.041.013261
TATCAAC43650.038.558462
TGGTATC10450.038.2314072
ATCAACG45050.037.4530833
TCAACGC45200.037.422824
CAACGCA45200.037.2347685
GGTATCA20150.036.9180831
AACGCAG47150.035.8751146
ACGCAGA51550.032.8079387
CGCAGAG52700.032.0920148
GCAGAGT57600.029.3619659
CAGAGTA42850.025.13921410-11
AGAGTAC40850.022.15705910-11
GAGTACG49250.021.52726412-13
AGAGTCG13000.020.5962945
GTATACT1909.580399E-720.1317184
GTACGGG53500.019.53991714-15
AGTACGG47300.018.95596312-13
TATAGTG2703.9981387E-918.8890175
Produced by FastQC (version 0.11.5)