FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457869
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.11077812889909863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40200.043.781681
TATCAAC44050.039.7511982
GTGGTAT11300.039.1266821
TCAACGC45000.038.624694
ATCAACG45300.038.3688963
CAACGCA45500.038.2936445
AACGCAG46600.037.480916
ACGCAGA50650.034.4815527
CGCAGAG52000.033.504648
TGGTATC13200.033.163642
GCAGAGT57550.030.3473599
GGTATCA21550.028.9993441
CAGAGTA44400.023.78306810-11
AGAGTAC42100.021.65058510-11
GAGTACG50900.020.53722412-13
AGTACGG46300.019.45715312-13
GTACGGG53000.019.44286714-15
GTATTAG5450.017.1610681
GTCTAGG3001.46847015E-817.0050581
GTATAAT3302.6211637E-916.7474041
Produced by FastQC (version 0.11.5)