FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457869
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27600.18931742152415615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36000.029.9524041
GGTATCA17800.026.7346341
TATCAAC40950.026.6539272
CAACGCA41600.025.7668765
TCAACGC41650.025.6681944
ATCAACG42400.025.34643
AACGCAG42650.025.0053526
GTGGTAT7600.024.2498661
AGAGTAC44050.023.9084811
ACGCAGA45100.023.8298077
CAGAGTA45000.023.46243710
CGCAGAG45800.023.405588
CGGGATA2250.023.22281617
TTAACGG600.00203386322.920435
AGTACGG46600.022.13039613
GCAGAGT49200.021.7918599
GAGTACG48950.021.1795912
TGGTATC8050.020.8333232
ACGGGAT9550.019.29368616
GTACGGG54300.018.68832414
Produced by FastQC (version 0.11.5)