FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812294
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20350.043.233811
TATCAAC23400.038.3204572
TCAACGC23900.036.9807784
CAACGCA24800.035.8100745
ATCAACG25000.035.523593
AACGCAG25650.034.7890476
ACGCAGA26500.033.5128257
CGCAGAG26650.033.164758
GGTATCA11200.032.636081
TGGTATC5350.030.9795362
GCAGAGT30700.028.791379
GTTTCGC600.00680223628.3352011
AGCGTCA1703.965397E-1027.4950433
GTGGTAT6050.026.6959741
GTATATA1502.78685E-622.6681611
CAGAGTA25550.022.61962310-11
CTAGGTT2006.679511E-821.2461684
AGAGTAC24200.020.6328810-11
ATTAGAC1254.7875728E-420.3963223
GAGTACG28500.020.27829412-13
Produced by FastQC (version 0.11.5)