FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812294
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20800.028.9642851
GTGGTAT4800.028.6556851
GGTATCA11100.024.7832951
TGGTATC5000.024.7432672
TCAACGC24200.024.5312234
ATCAACG24300.024.3186663
TATCAAC24800.024.166812
CAACGCA24650.024.0848735
AACGCAG25100.023.6530766
GATCGAC952.3151097E-623.15729920
TCGACAC952.3151097E-623.15729922
ACGCAGA25650.023.1458937
AGAGTAC25650.023.04866811
CAGAGTA26350.022.53794910
CGCAGAG26650.022.0711068
GTCTTAT650.003232488621.1611211
GCAGAGT28250.021.020839
AGTACGG29950.019.83376513
GAGTACG30700.019.16888812
GTTAGCC1301.6425893E-619.0285953
Produced by FastQC (version 0.11.5)