FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509600
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43820.2902755696873344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8650.032.741522
GTGGTAT9350.030.9019991
GTATCAA42100.029.2822721
GGTATCA19300.026.0917151
TATCAAC46800.025.9689352
TCAACGC48600.024.6586724
ATCAACG49100.024.5203423
CAACGCA49750.024.2007735
AACGCAG51000.023.8240016
AGAGTAC49950.023.23029511
ACGCAGA52400.023.1350237
CGCAGAG52650.022.7641148
CAGAGTA52500.022.25466210
GCAGAGT56450.021.2331269
AGTACGG56050.020.50995813
GAGTACG58000.019.91259412
TACGGGG22850.019.37776815
GTACTAT2700.019.364161
TACGCGA1008.999192E-519.24756629
CTGCCGA8350.018.44811655
Produced by FastQC (version 0.11.5)