FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273312
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG505.454638E-542.4969185
GTATCAA27100.040.3157541
GGTATCA14850.039.5060461
TATCAAC29150.036.8899082
ATCAACG29900.035.81683
CAACGCA29950.035.7570045
TCAACGC30250.035.402394
AACGCAG30500.035.1122066
GTGGTAT7550.034.910471
TGGTATC7550.033.777692
ACGCAGA32850.032.59917
CGCAGAG36250.029.4242958
GCAGAGT40500.026.756279
CAGAGTA31650.023.89938210-11
ATACCGT1203.6335926E-421.2484596
GAGTACG37800.019.95477912-13
AGAGTAC29700.019.67372510-11
ACGGGAT7250.019.63650716-17
GTACGGG38450.019.396414-15
GTATAGA2302.9026887E-718.4834541
Produced by FastQC (version 0.11.5)