FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273312
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17230.1353164032067553No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5150.033.6564751
GGTATCA12350.028.0697731
TGGTATC6000.027.4944612
GTATCAA25650.027.4592041
TATCAAC29000.024.2709732
TCAACGC29650.023.6405934
ATCAACG29850.023.4821973
CAACGCA30050.023.3277425
AACGCAG31050.022.4011346
ACGCAGA32350.021.5859187
CGCAGAG33000.021.2440518
AGAGTAC31800.021.09969311
CAGAGTA33000.020.49748210
TGCCGAC4300.019.82937855
GCAGAGT37350.018.8456739
AGTACGG36050.018.61440713
TACGGGG17900.018.28418715
GAGTACG37700.017.72537412
CGAACGG1354.800172E-516.29813614
GTACGGG41300.016.24880614
Produced by FastQC (version 0.11.5)