FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455184
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA200.003222693763.7476925
TGGTATC4050.043.0278632
GTGGTAT4250.041.012031
GTATCAA13450.039.5097351
TATCAAC14800.035.3235472
TCAACGC15050.034.1681984
GGTATCA7300.033.7770351
ATCAACG15400.033.6676143
CAACGCA15350.033.2235535
AACGCAG15850.032.175496
ACGCAGA17350.029.3937517
CGCAGAG17650.029.3757088
TTAGCGT905.14965E-528.3323084
GCAGAGT18400.027.947369
TACGATT1601.8268838E-723.9053847
GCTTAAG900.001706566123.618041
AGAGTCG3100.023.3056095
CAGAGTA15050.022.87292710-11
CGATTCG1703.2790558E-722.4991869
GTATATG950.002336961522.3749871
Produced by FastQC (version 0.11.5)