FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455184
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7440.16345038489929348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATT403.9291626E-641.25832414
GTATCAA11000.028.7526441
GGTATCA5250.028.2883131
CGAATTA400.00786047427.50554815
ATGCGAC400.00786469127.50252740
CTATACT606.3244705E-527.4904424
TATCAAC12150.026.2575592
TGCCGAC1700.025.8932755
TGGACCG651.0901208E-425.3757935
AGGACCG550.001227902124.9913125
TGGTATC2550.024.8062022
ATCAACG13350.024.0980433
TCAACGC13550.023.9400184
CAACGCA13600.023.8520035
AACGCAG13750.023.5917996
GTGGTAT2800.023.5735951
CGCAGAG14300.022.492188
AGAGTAC13900.022.35325811
ACGCAGA14750.021.9923557
CAGAGTA14500.021.8051610
Produced by FastQC (version 0.11.5)