FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900320
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17200.19104318464545939No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.13128665363426337No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT9870.10962768793317931No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28750.044.8063281
GGTATCA14100.040.4036141
TATCAAC32900.039.4019622
GTGGTAT6800.038.7629931
TCAACGC34050.038.1896674
ATCAACG34100.038.0090453
TGGTATC6600.037.994612
CAACGCA34650.037.6510165
AACGCAG35400.036.973376
ACGCAGA38450.034.0404977
CGCAGAG40100.032.6398288
GCAGAGT42850.030.4459199
ACACCGT1106.4997294E-627.0425036
CAGAGTA31250.026.10987510-11
AGCGTCA2051.2914825E-1024.8753343
GAGTACG34050.023.46423512-13
AGAGTAC28900.023.15988210-11
ATCGGTC950.002344201322.365988
GTACGGG36700.021.48045714-15
AGTACGG31200.021.45317612-13
Produced by FastQC (version 0.11.5)