FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900320
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22740.2525768615603341No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5450.033.2931982
GTGGTAT5700.031.3785421
GTATCAA26950.028.997051
CGTCTAA606.309488E-527.50433540
GCTATCG400.00787045427.5012817
GGTATCA12600.026.2062551
TATCAAC30350.025.8163172
ATCAACG31200.025.1101973
CGGTCGT550.001227604924.99560510
TCAACGC31500.024.8710524
CAACGCA31950.024.608165
AACGCAG32850.024.0176496
AGAGTAC30800.023.83509411
ACGCAGA33700.023.4934377
CGCAGAG33750.023.2142738
CAGAGTA32250.022.84869610
TGCCGAC3150.022.7019955
CGGACGT852.5663927E-522.65062736
GCAGAGT36550.021.4370789
AGTACGG33800.021.31755813
Produced by FastQC (version 0.11.5)