FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423706
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10270.24238505001109262No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGG300.00192145436.67753254
CGATCGT300.001922568736.67320347
CCGATCG300.00192368436.6688746
TGGTATC2450.033.6635672
GTGGTAT3100.028.3955121
GTATCAA13300.027.714981
GTATAGA507.012377E-427.5081521
CGTAGAA400.00786530427.50165633
CCTAGCG507.022068E-427.50165417
CGAGACT400.0078925427.4821824
GGTATCA6600.027.091361
TATCAAC14300.025.9539052
CGGGATA759.728925E-625.6682117
AGCGATA651.08692984E-425.3861420
GCGATAG550.001224845625.00150521
CGATAGG550.001224845625.00150522
TCAACGC14900.024.7155174
ATCAACG14950.024.6357653
CAACGCA15250.024.1511255
AACGCAG15250.023.9708946
Produced by FastQC (version 0.11.5)