FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098095
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13840.12603645404086167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30150.042.7139131
TATCAAC33400.038.6778262
TCAACGC35050.037.0961464
ATCAACG35300.036.7130553
GTGGTAT9150.036.6961141
CAACGCA36100.036.1348765
AACGCAG36700.035.8914536
CCGTATG751.4858149E-533.9926769
GGTATCA15700.033.568821
ACGCAGA39350.033.3663867
TGGTATC10500.032.376932
CGCAGAG42000.030.9576198
ACCGTAT1003.099438E-629.7435918
GCAGAGT44600.029.4387269
CAGAGTA32250.025.23110-11
AGAGTAC31700.022.25072910-11
GTATTAA4200.021.2512281
GAGTACG37850.021.16122812-13
AGTACGG34350.020.22490112-13
GTACGGG39800.020.07105414-15
Produced by FastQC (version 0.11.5)