FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098095
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11860.10800522723443781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32500.030.5628641
GTGGTAT6900.027.9138131
TATCAAC36400.027.426952
ATCAACG36300.027.4117533
TGGTATC7100.027.1151472
TCAACGC37400.026.6055264
CAACGCA38200.026.1226795
AACGCAG38350.025.8054566
GGTATCA15400.025.3710161
ACGCAGA39100.025.3104677
AGAGTAC38350.025.0932111
CGCAGAG39500.025.0553028
CAGAGTA39550.024.81735410
GCAGAGT42550.023.4552929
AGTACGG42350.022.59740313
GAGTACG45050.021.36320312
TACGGGG20150.020.33591315
GTACGGG48100.019.89695514
ACGGGAT8350.018.77416616
ACGGGGC5750.018.65387316
Produced by FastQC (version 0.11.5)