FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502219
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA5570.11090779122255431No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA5550.11050955857902628No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT5520.10991220961373425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16050.045.8241921
TATCAAC18000.040.8558352
GGTATCA7950.040.1067921
AACGCAG18550.039.8696676
CAACGCA18500.039.7476655
TCAACGC18550.039.640534
ATCAACG18800.039.1133963
ACGCAGA19750.037.4397477
TGGTATC3250.036.6231082
CGCAGAG20900.035.176348
GTGGTAT3550.034.7290881
GCAGAGT22150.033.383079
GTACCTA853.4843215E-530.003346
GTTATAA600.00679168328.342131
CAGAGTA18450.027.06401610-11
TAGGAGT1606.2209438E-926.5654564
AGAGTAC18000.022.42858510-11
GTGTAAG1553.6917227E-621.9422951
GAGTACG23100.021.43209812-13
GCTTGTC2601.07320375E-1021.2565981
Produced by FastQC (version 0.11.5)