FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502219
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5720.11389453604901448No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17850.030.9916441
GTGGTAT3650.030.9156061
TGGTATC3800.029.671592
TATACTA507.0461043E-427.4868075
TATCAAC20250.027.4325962
TCAACGC20350.026.8816684
CAACGCA20700.026.690095
ATCAACG20600.026.558083
AACGCAG21150.026.1222136
ACGCAGA21700.025.460137
CAGAGTA21300.025.42713510
AGAGTAC21250.025.10133711
GGTATCA8350.025.0503561
CGCAGAG22100.024.9993158
GCAGAGT23400.023.6128149
CGCACGC903.9862418E-521.39140715
TTAAGAC903.9985098E-521.3828873
TTATACT904.0015817E-521.3807564
AGTACGG25050.020.85866213
TGCCGAC1601.3315002E-920.62742855
Produced by FastQC (version 0.11.5)