FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644149
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28430.17291620163379354No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20980.12760400669282407No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT17290.10516078530595464No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14900.042.5071331
GTATCAA55600.042.354231
TGGTATC14500.042.206282
GGTATCA28900.039.2712941
TATCAAC62400.037.323172
TCAACGC65750.035.4204564
ATCAACG65700.035.2533573
CAACGCA65950.035.2486045
AACGCAG69650.033.6201676
ACGCAGA74900.031.1491857
CGCAGAG75050.031.0869278
GCAGAGT80600.028.9463279
CAGAGTA58900.026.01113710-11
GAGTACG66100.022.9849712-13
GTACGGG69150.021.75607114-15
AGAGTAC56850.021.4172410-11
ACGGGAT13350.020.85080316-17
ACGATAG1050.004188500320.2371835
AGAGTCG19050.019.1854885
AGTACGG63500.019.10740712-13
Produced by FastQC (version 0.11.5)