FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641945
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24540.14945689411033866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10950.035.657722
GTGGTAT12350.033.4221571
GTATCAA56350.028.9093251
GGTATCA25600.027.6250691
TATCAAC63750.025.5341172
TCAACGC64550.024.741554
ATCAACG65300.024.542323
CAACGCA65900.024.444015
AGAGTAC64850.024.16819211
AACGCAG67700.023.7549386
CAGAGTA67000.023.55393410
CGCAGAG68650.023.3067848
ACGCAGA69850.022.9850987
CGCGTAG752.869855E-422.00399834
GCAGAGT74400.021.6176919
TACGCGA904.0026407E-521.38495429
AGTACGG72550.021.37833613
TACGGGG29650.021.05522315
GAGTACG75200.020.8071912
ACGCGTA856.6787715E-419.41470133
Produced by FastQC (version 0.11.5)