FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894491
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29700.3320324072573117No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22240.24863302146136743No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15070.16847570294167297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9100.052.7759061
TGGTATC10250.046.434872
GTATCAA35100.041.4111861
TATCAAC38650.037.1635552
TCAACGC41450.034.8581624
CAACGCA41600.034.7324685
ATCAACG41750.034.607683
GGTATCA19200.034.532021
AACGCAG43400.033.389876
ACGCAGA48200.030.0647357
CGCAGAG49400.029.3344218
GCAGAGT52200.027.6795189
CAGAGTA33500.024.99030110-11
GTATAAC900.001710546923.6116391
GAGTACG37150.022.477812-13
AGAGTAC32250.021.54458410-11
ACGGGAT8100.020.46113216-17
GTACGGG41250.020.03760114-15
TACGAAG1507.1485614E-519.831562
ACGGGTC1950.019.6136316-17
Produced by FastQC (version 0.11.5)