FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712531
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34480.48390877028508233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5250.036.6479032
GTGGTAT5200.035.96081
GTATCAA26050.030.9302711
TATCAAC29150.027.7216552
GCGTAGT400.0078719427.49943711
ATCAACG31200.025.6341133
TCAACGC30750.025.5605564
CAACGCA31100.025.3666085
AACGCAG31650.025.1898826
AGAGTAC30200.024.85876311
CAGAGTA30800.024.46378710
ACGCAGA32800.024.30677
CGCAGAG32700.024.2986668
GGTATCA11700.023.973871
CGGGATA1402.5102054E-1023.57425917
GCAGAGT34650.023.0154049
GTCGAGG752.8702046E-422.00109514
AGTACGG34800.021.41629613
TGCCGAC3100.021.29437455
GTCTACG650.00323893921.1534141
Produced by FastQC (version 0.11.5)