FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755804
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20750.27454207704643No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15420.2040211483400458No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10240.13548486115447922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7950.049.7185331
TGGTATC8150.046.927772
GTATCAA28150.040.161111
TATCAAC31200.036.7751242
GGTATCA14500.036.6390721
TCAACGC32900.034.7434234
ATCAACG33100.034.5334933
CAACGCA33700.034.2969325
AACGCAG34750.033.0182386
CGCAGAG39050.029.4912538
ACGCAGA39350.029.2664157
GCAGAGT42850.026.9768839
GTCTATA850.001226889624.997472
CAGAGTA26700.024.83059110-11
GAGTACG29850.021.92480512-13
GCTTATA1404.1979707E-521.2506621
TACTAGA1000.003156524421.247852
AGAGTAC25250.020.87055610-11
GTACTAT1254.7803263E-420.4006371
GTACGGG32100.020.18876614-15
Produced by FastQC (version 0.11.5)