FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755804
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12260.16221136696815577No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5500.042.0194661
TGGTATC6300.036.227762
GTATCAA27350.028.3678051
TATCAAC30350.025.45962
GGTATCA13300.025.444121
AGAGTAC30850.024.7746411
CAACGCA31600.024.6200165
AACGCAG32050.024.4442716
ATCAACG31550.024.397633
TCAACGC31650.024.3173274
CAGAGTA31550.024.31049510
CGCAGAG32850.023.7668698
ACGCAGA33400.023.4578067
GCAGAGT35650.022.1315279
AGTACGG34350.021.85878813
GAGTACG35750.021.22795912
GTCTAAT650.003230041621.1636521
CGGGCGA700.00497067919.64545617
CGAATTA700.00497067919.64545615
GTACGGG38650.019.21341114
Produced by FastQC (version 0.11.5)