FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906218
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15290.16872319905364935No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATA300.001925275436.66718323
TGGTATC5900.034.948472
GTGGTAT6250.032.5622561
GTATCAA33350.030.5943221
GGTATCA15350.028.1289831
TATCAAC37450.027.3092692
ATCAACG39100.025.8755763
TCAACGC39300.025.6739354
CAACGCA40000.025.223255
AGAGTAC39250.024.8001611
AACGCAG41150.024.6546846
CGCAGAG41800.024.1397468
CAGAGTA40650.024.01367810
ACGCAGA42400.023.8629937
GCAGAGT44500.022.8011769
ACGGGGC3600.022.1530916
AGTACGG44250.021.44097913
GAGTACG45150.021.25604612
TACGGGG17650.021.190115
ACTACCG804.4375463E-420.62756737
Produced by FastQC (version 0.11.5)