FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975429
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25150.030.4124431
TCAACGC28850.026.2983914
ATCAACG29200.026.0786483
CAACGCA29400.025.994755
GGTATCA11450.025.9515171
TATCAAC29900.025.841182
CGAACTA1600.025.78060229
AACGCAG29750.025.5991486
ACGCAGA31050.024.5273637
CGCAGAG31100.024.4007788
AGAGTAC30500.024.25224511
CAGAGTA31650.023.89110410
TGGTATC5150.022.9582332
GATATAC600.002039252322.909043
GTGGTAT5700.022.203811
GCAGAGT34350.021.9331539
TGCCGAC3700.021.55682855
AGTACGG33600.021.52586213
GAGTACG35050.020.8685912
GTATACC700.004961488319.6524411
Produced by FastQC (version 0.11.5)