FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348771
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32270.23925484756122428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8050.033.493011
TGGTATC8550.030.229292
GGTATCA19050.028.3064291
GTATCAA39050.028.1109711
TAAGACG606.341148E-527.4855964
TATCAAC43400.025.2783892
TCAACGC44800.024.3566554
ATCAACG44700.024.289973
CAACGCA45150.024.1696365
AACGCAG45550.024.018636
ACGCAGA47250.023.2126437
CGCAGAG47150.023.146138
AGAGTAC47600.022.47113411
CAGAGTA49250.021.82833710
GCAGAGT51200.021.2654889
TGCCGAC6800.021.03399355
AGTACGG52400.020.15257613
TTAGTCG700.004970207319.64713752
TAGTCGT856.6749315E-419.41599353
CGGGATA2157.2759576E-1219.18880317
Produced by FastQC (version 0.11.5)