FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630128
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18420.11299726156473602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44550.045.6104051
TATCAAC49600.040.8721242
TCAACGC51100.039.5855374
ATCAACG51700.039.2905273
TGGTATC11450.038.9739382
CAACGCA52050.038.9446835
GTGGTAT11650.038.3130871
GGTATCA21750.037.9163251
AACGCAG54900.036.9229626
CGCAGAG58300.034.767528
ACGCAGA58700.034.4582147
GCAGAGT64600.031.376889
CAGAGTA50600.024.98390810-11
AGAGTAC48150.021.88664810-11
TATACTG4100.021.7663695
GAGTACG57850.021.66918412-13
GTACGGG59800.020.6428114-15
AGTACGG51950.020.0812112-13
CGTCAGA3500.019.4268535
TACGGGG22700.017.59648314-15
Produced by FastQC (version 0.11.5)