FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630128
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25250.15489581186262674No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9200.034.072932
GTGGTAT9750.033.012141
GTATCAA43300.028.5266551
GGTATCA20300.027.1035611
TATCAAC48250.025.759272
TCAACGC49850.024.5985134
CAACGCA50700.024.2945735
ATCAACG50950.024.123623
AACGCAG52200.023.649126
TGCCGAC5250.023.05114455
AGAGTAC52650.022.92760311
CGCAGAG53900.022.8019128
ACGCAGA54350.022.71367
CAGAGTA55050.022.12783410
GCAGAGT58650.021.0040259
AGTACGG58350.020.54840513
GAGTACG61550.019.43361912
TACGGGC8800.018.12554715
ACGGGAT12950.018.05073716
GTACGGG65900.017.98612614
Produced by FastQC (version 0.11.5)