FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884199
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC5650.044.3867342
GTATCAA24450.041.209071
GGTATCA13200.040.2586671
GTGGTAT6150.040.0937081
TATCAAC27350.036.6778832
ATCAACG28200.035.7210583
TCAACGC28600.035.2214624
CAACGCA29100.034.762345
AACGCAG30450.033.2211536
ACGCAGA30850.032.7848477
CGCAGAG32000.031.341048
GCAGAGT34150.029.4923239
CAGAGTA30050.023.97047210-11
GTATATA1502.7814276E-622.6736811
GTATTAT1703.2753815E-722.5069641
GAGTACG33650.021.4691712-13
CGATCGT500.00315273321.25417372-73
GTACGGG35100.020.40065814-15
AGAGTAC29500.019.87963710-11
CCGTCTT10150.019.0522384-85
Produced by FastQC (version 0.11.5)