FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884199
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16250.18378215763645966No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCG453.7912533E-430.56049250
GTATCAA22800.030.294441
GGTATCA10950.029.403281
TATCAAC25800.026.8663232
TGGTATC4100.026.835122
GTGGTAT4050.026.4992521
CAACGCA27150.025.3149155
ATCAACG26950.025.3016193
TCAACGC26950.025.2987564
AACGCAG28050.024.5040596
ACGCAGA28350.024.2447577
AGAGTAC28800.023.67768311
CGACGAC1052.0941116E-723.57523555
CGCAGAG29350.023.41878
CAGAGTA29400.023.3788710
CGAATCG852.5673713E-522.64943516
CTATCGG852.5707408E-522.64559212
GCAGAGT31150.022.2419749
TAACGTG752.8753898E-421.99608628
AGTACGG31300.021.70355613
Produced by FastQC (version 0.11.5)