FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052674
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.140309345533375No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8500.044.510871
GTATCAA31450.042.983471
CCGGTCG409.325285E-442.494239
TGGTATC8950.041.3111652
TATCAAC35850.037.1044852
TCAACGC36200.036.8613854
CAACGCA36500.036.325565
ATCAACG37050.036.0157133
GGTATCA16750.035.784861
AACGCAG37600.035.2628446
ACGCAGA41000.032.544367
CGCAGAG41000.032.4407168
GCAGAGT46300.028.818989
CAGAGTA35900.023.91096910-11
CCATACG450.001712415723.60902614-15
TATACTG2053.4724508E-922.8028645
GAGTACG38600.021.90868812-13
AGAGTAC32550.021.6066410-11
TACGCGA1209.240466E-1021.24913428-29
GTACGGG39450.021.16733214-15
Produced by FastQC (version 0.11.5)