FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843986
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10770.12760875180393988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30900.043.8783721
TGGTATC8550.039.7640572
GTGGTAT8850.038.4206771
TATCAAC35800.037.5120542
GGTATCA17250.037.451811
TCAACGC37950.035.612954
CAACGCA38050.035.4076545
ATCAACG38350.035.128593
AACGCAG38400.034.9742476
CGCAGAG41500.032.4641278
ACGCAGA41500.032.4622047
GCAGAGT45100.029.8727559
CAGAGTA35950.025.29848910-11
AGAGTAC35200.022.03433210-11
CCGTCTT8300.022.01825784-85
GAGTACG40850.022.00316612-13
AGAGTCG8350.021.886415
GATCGAC3150.021.58620520-21
CTATACT1909.572213E-720.1317484
GTACGGG44750.019.9900114-15
Produced by FastQC (version 0.11.5)