FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843986
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19440.23033557428677726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31400.031.2895981
GGTATCA13750.031.2236251
TATCAAC35400.027.347492
TCAACGC36250.026.462944
ATCAACG36500.026.3601193
CAACGCA37050.025.9641915
AACGCAG37900.025.4604346
AGAGTAC38350.025.4514411
CGCAGAG38250.025.2993378
CAGAGTA38850.025.19166410
ACGCAGA38450.025.0962417
TATACGT550.001229779124.9877994
GTGGTAT5300.024.9245191
TGGTATC5450.024.2227152
GCAGAGT41150.023.6500119
TCGACAC2700.022.40576622
TACGGGG19550.021.8053515
AGTACGG44150.021.80166413
GGATCGA2950.021.44164819
GAGTACG45450.021.41757212
Produced by FastQC (version 0.11.5)