FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525197
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA250.00778428251.0159451
GTATCAA16200.045.9248471
GTGGTAT4200.042.513291
TGGTATC4300.040.520492
TATCAAC18750.039.663952
ATCAACG18800.039.5584643
TCAACGC19150.039.0573844
CAACGCA19200.038.7343335
AACGCAG20050.037.0922286
GGTATCA9100.036.439961
CGCAGAG21100.035.2464078
ACGCAGA21300.034.9154557
GCAGAGT23000.032.3347479
GTAAGAC1003.0918363E-629.7479653
ATATCGC600.00680453728.3313986
GTGTAAG1255.674956E-727.2085041
TAGGCCC808.6086284E-426.5606845
CAGAGTA20350.024.84851510-11
GCTTATA1302.3692728E-522.883052
AGAGTAC19450.021.95656410-11
Produced by FastQC (version 0.11.5)