FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682585
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20000.11886472303033724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39950.031.6798741
GGTATCA18350.028.7877371
TATCAAC44800.028.2342872
TCAACGC46950.026.7584824
ATCAACG47150.026.6457673
CAACGCA47550.026.3630225
AACGCAG49850.025.0922746
GTGGTAT7300.024.4981271
ACGCAGA51450.024.3661067
CGCAGAG51700.024.30298
AGAGTAC51200.024.11423111
CAGAGTA51950.023.87124610
GCAGAGT56000.022.3903529
AGTACGG57050.021.498213
TGGTATC8400.020.9506592
GAGTACG59550.020.68741412
TACGGGG26850.019.76736515
GTACGGG64100.018.87632814
ACGGGAT12250.017.7348216
ACGGGGG10300.017.62148316
Produced by FastQC (version 0.11.5)