FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054694
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.16829525909884763No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.11244967734717368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30600.042.097121
TGGTATC8850.041.7814562
GTGGTAT9250.040.9052851
TATCAAC33550.038.511342
ATCAACG35850.035.9186363
TCAACGC36150.035.620564
GGTATCA14750.035.1640551
CAACGCA36850.035.059245
AACGCAG37850.034.469816
CGCAGAG42150.030.8510278
ACGCAGA42350.030.8042187
TACCGGA905.1532166E-528.334552
GCAGAGT47800.027.1155099
CAGAGTA33950.023.65744410-11
ACGGGAT7450.022.81652616-17
AGAGTAC31450.022.02484110-11
AGAGTCG12400.021.5916215
GAGTACG39100.020.43323712-13
GTACGGG39350.020.08742714-15
AGTCGAC13800.019.7072547
Produced by FastQC (version 0.11.5)