FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040701
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT17280.16604192750847746No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.11146333096633902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7850.042.783131
GTATCAA30500.041.536661
TGGTATC8050.040.6522642
GGTATCA15200.036.6389351
TATCAAC34900.035.924152
TCAACGC35750.034.5944824
CAACGCA36500.033.883645
ATCAACG37800.032.9431953
AACGCAG38850.031.9434436
ACGCAGA41350.030.0092757
CGCAGAG41850.029.6507438
GCAGAGT44150.028.3948429
CAGAGTA35350.023.20150210-11
GAGTACG37550.021.50264212-13
AGAGTAC32700.021.18302710-11
GTACGGG40250.020.1658114-15
GACAGGT5600.018.978731
CTGTTCG3156.91216E-1118.8871159
AGTACGG37050.018.40920412-13
GCTTGTA4200.018.219581
Produced by FastQC (version 0.11.5)