FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040701
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39830.38272279934390374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5700.035.7046551
TGGTATC6150.031.2928262
GGTATCA15250.029.2154921
GTATCAA31850.028.2362021
CGGGGTA1650.026.66883517
TATCAAC35850.025.2306582
TCAACGC36500.024.247094
ATCAACG37000.024.2177263
CAACGCA37500.023.9669695
AACGCAG38000.023.7239446
AGAGTAC37550.023.28636411
ACGCAGA38800.023.2347897
CGCAGAG38950.023.0758558
CAGAGTA38500.022.99413110
GCAGAGT41150.021.9131589
AGTACGG40800.021.03112213
GAGTACG42250.020.50166712
CTGCCGA7500.019.80637255
GTATAGT1401.5284968E-719.6444531
TACGGGG18300.019.38682415
Produced by FastQC (version 0.11.5)