FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154636
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35020.3032990483580973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6600.032.5080681
TGGTATC6600.031.6608072
GTATCAA35300.030.2341331
GGTATCA16450.029.7642271
CCGTCCG507.0413336E-427.4937226
TATCAAC39800.026.9422472
TCAACGC40400.026.328824
ATCAACG40950.026.0468273
CAACGCA41600.025.5032655
AACGCAG43600.024.6517936
CGCAGAG43550.024.4907348
AGAGTAC42200.024.2382711
ACGCAGA45000.023.8848467
CAGAGTA44100.023.37810
TGCCGAC3600.022.92334755
CGAATTG600.002034648622.91838520
ACGGGAT9900.022.7804716
GCAGAGT48000.022.3376799
AGTACGG46600.021.71742413
ACGGGTA1901.8189894E-1221.71309316
Produced by FastQC (version 0.11.5)