FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859227
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60840.7080783075950826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22650.031.8274361
GTGGTAT4800.027.514941
TATCAAC26450.027.0342252
TCAACGC26600.026.462054
ATCAACG27350.026.1446173
CAACGCA27050.026.0172865
TGGTATC5050.025.5960372
AACGCAG27650.025.3562436
TAGCACG550.001226582424.9990220
ACGCAGA28350.024.8271457
CGCAGAG28500.024.6964748
GGTATCA10550.024.2548751
AGAGTAC28500.024.2183511
CAGAGTA28800.024.15563610
ATAGAAC952.3132998E-623.1596833
GCAGAGT30850.022.8165459
TATACTG1155.05599E-721.5146355
AGTACGG32200.021.26593213
TGCCGAC3950.020.88890555
GAGTACG33600.020.46050812
Produced by FastQC (version 0.11.5)