FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26457
Sequences flagged as poor quality0
Sequence length91
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA501.6370905E-1068.01
GTGCTTG200.00318876163.751
AGTAGCG250.007712804751.05
CTGGGGT250.007712804751.09
GCAGGGA250.007712804751.06
GTGGAAG250.007712804751.09
GTATCAA851.808985E-840.02
AACGCAG954.8210495E-835.7894747
CAACGCA954.8210495E-835.7894746
TCAACGC1007.57027E-834.05
TATCAAC1007.57027E-834.03
ATCAACG1007.57027E-834.04
GCCGTCT1150.029.56521684-85
ACGCAGA1203.7471546E-728.3333328
CAGAGTA1150.027.7173910-11
CGCAGAG1255.3548865E-727.1999999
GTACGGG1400.022.76785714-15
GCAGAGT1353.025326E-522.0370379
GAGTACG1501.8189894E-1221.2512-13
ATGCCGT1700.021.2582-83
Produced by FastQC (version 0.11.5)