FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26457
Sequences flagged as poor quality0
Sequence length61
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390.14740900328835468No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGACT150.00572156355.019
ATTATCC150.00572156355.023
TGCCGAC257.717643E-444.00000455
CTGCCGA300.001891852936.66666854
GTATCAA801.3882527E-830.9375021
GCTGCCG801.3882527E-830.93750255
TATCAAC852.5045665E-829.117652
TCAACGC1001.2118653E-724.7500024
AACGCAG1051.9423351E-723.5714286
ACGCAGA1051.9423351E-723.5714287
ATCAACG1051.9423351E-723.5714283
GCAGAGT952.1772103E-623.1578949
CGCAGAG952.1772103E-623.1578948
CAACGCA952.1772103E-623.1578945
CGCTGCC1103.0432057E-722.554
CAGAGTA903.8092847E-521.3888910
AGAGTAC955.7783196E-520.26315911
AGTACGG1008.572813E-519.25000213
GTACGGG1051.2466646E-418.33333414
GAGTACG1101.7802861E-417.512
Produced by FastQC (version 0.11.5)