FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221086
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCT2470.11172123065232534No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA2270.10267497715820992No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTGC200.003221328863.7435536
GTATCAA6500.051.017921
TATCAAC7500.044.195532
TAGTAGT409.3076844E-442.4957054
GGTATCA2850.041.769061
CAACGCA7950.041.159365
ATCAACG8000.040.9021153
AACGCAG8050.040.6480646
TCAACGC8050.040.6480644
ACGCAGA8450.038.7238967
GTGCTAT559.517072E-538.649941
TGGTATC1101.10958354E-1038.6324582
CGCAGAG8550.038.2709858
TGGACCG450.001660966937.7739565
GTCGTTG301.5948922E-435.41308648-49
GCAGAGT9500.034.891219
AGATCCG550.00444251530.9059645
TATTGGA550.00444251530.9059642
GTTTTAT600.00678222328.343291
TGGAACG600.00679723128.3304675
Produced by FastQC (version 0.11.5)