FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25543
Sequences flagged as poor quality0
Sequence length61
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG730.2857925850526563No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACCC150.005719204455.07
GGTATCA351.5118676E-647.1428571
TGCCGAC501.751549E-533.055
ACAGGGA453.69342E-430.5555573
GTATCAA752.759134E-729.3333321
TGGGGCT400.00773201327.543
TATCAAC804.853591E-727.52
CACAGGG400.00773201327.52
CTGCCGA651.0486597E-425.38461554
TCAACGC1003.3915294E-622.04
GCTGCCG752.767475E-422.053
AGAGTAC1003.3915294E-622.011
ATCAACG1003.3915294E-622.03
TACGGGA650.003154758621.15384715
AACGCAG1055.177706E-620.9523816
GCAGAGT1055.177706E-620.9523819
CAACGCA1055.177706E-620.9523815
ACGCAGA1107.7447385E-620.07
CAGAGTA1107.7447385E-620.010
AGTACGG1107.7447385E-620.013
Produced by FastQC (version 0.11.5)