FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005412791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27662
Sequences flagged as poor quality0
Sequence length61
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1450.5241848022558022No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTACA150.00572431255.02
GAAAAGG150.00572431255.018
GTACAGA150.00572431255.04
GTATCAA708.54925E-1139.2857131
TCAACGC856.9303496E-1032.352944
TATCAAC856.9303496E-1032.352942
GGTGGCC350.004031439331.4285723
AACGCAG901.2769306E-930.5555576
ATCAACG901.2769306E-930.5555573
ACGCAGA952.2773747E-928.9473697
AGAGTAC952.2773747E-928.94736911
CAACGCA952.2773747E-928.9473695
AGTACGG1003.938112E-927.50000213
CGCAGAG1003.938112E-927.5000028
GTTTTGT400.00774317327.51
ACGGGAT400.00774317327.516
GAGTACG1056.6283974E-926.19047712
CAGAGTA1101.0877557E-825.00000210
TACGGGA701.7395393E-423.57142815
GTACGGG1202.7417627E-822.91666614
Produced by FastQC (version 0.11.5)