FastQCFastQC Report
Fri 27 May 2022
EGAF00005412793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005412793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159647263
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT7007860.43895898171458164Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT6695670.41940399567012937Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6012420.37660651908576726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA4817100.30173395456206475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3283000.2056408571188596Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2830150.17727519700729225Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT2613290.1636915003046435No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2605040.16317473604292235No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2448120.15334556659452409No Hit
CACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCC2322930.1455039038157516No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG2111700.13227285957291984No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC2075890.1300297894865883No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG2020050.12653207841089015No Hit
GTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAAC1925560.1206134050666437No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC1894870.11869104201304097No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC1865080.11682505324253507No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1776400.111270307214725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACC1771050.11093519342076036No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC1759010.1101810307891091Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAAT1750980.1096780469077005No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA1730940.10842277953741054Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT1725600.1080882921243692No Hit
CTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAG1720110.1077444089974784No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1640240.1027415045630942No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA1631610.1022009378262877No Hit
AATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAA1622210.1016121397583872No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1606650.10063749104173493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGATAAAATTTGAAATC1600810.10027168458252866Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)